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CAS No.: 103404-75-7

D-萤光素钠盐 Catalog No. CSN10016

Synonyms: D-(-)-Luciferin sodium;Firefly luciferin sodium;Beetle Luciferin sodium

D-Luciferin sodium is a substrate for firefly luciferase with a Km of approx 2 μM. D-luciferin can be employed to assay the expression of the luciferase gene linked to a promoter of interest. Alternatively, D-luciferin and luciferase can be used to assess ATP availability in cellular or biochemical assays.

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技术信息

生物活性

描述D-Luciferin sodium is a substrate for firefly luciferase with a Km of approx 2 μM. D-luciferin can be employed to assay the expression of the luciferase gene linked to a promoter of interest. Alternatively, D-luciferin and luciferase can be used to assess ATP availability in cellular or biochemical assays.
Procedure^[3]:
1. Prepare a fresh stock solution of Luciferin at 15mg/mL in DPBS. Filter sterilize through a 0.2 µm filter.
2. Inject 10 µL/g of body weight. Each mouse should receive 150 mg Luciferin/kg body weight. (e.g. For a 10 g mouse, inject 100 µL to deliver 1.5 mg of Luciferin.)
3. Inject the Luciferin intra-peritoneally (i.p.) 10-15 minutes before imaging.
Note: A Luciferin kinetic study should be performed for each animal model to determine peak signal time.
参考文献

[1] Schoellhammer CM, Lauwers GY, et al. Ultrasound-Mediated Delivery of RNA to Colonic Mucosa of Live Mice. Gastroenterology. 2017 Apr;152(5):1151-1160.
[2] Patrick PS, Lyons SK, et al. Oatp1 enhances bioluminescence by acting as a plasma membrane transporter for D-luciferin. Mol Imaging Biol. 2014 Oct;16(5):626-34.
[3] Preparation of Luciferin for In Vivo Bioluminescent Assays

实验方案

计算器摩尔计算器总药量计算器工作液计算器
储存液制备
- 1 mg
- 5 mg
- 10 mg
- 1 mM
- 3.3080 mL
- 16.5399 mL
- 33.0797 mL
- 5 mM
- 0.6616 mL
- 3.3080 mL
- 6.6159 mL
- 10 mM
- 0.3308 mL
- 1.6540 mL
- 3.3080 mL

技术信息

CAS号

103404-75-7

储存条件

粉末: Inert atmosphere,Store in freezer, under -20°C

液体: -20°C:3-6 月 -80°C:12月

分子式

C11H7N2NaO3S2

运输

蓝冰

分子量

302.30

别名

D-(-)-Luciferin sodium;Firefly luciferin sodium;Beetle Luciferin sodium;D-(-)-Luciferin Sodium Salt;Firefly Luciferin Sodium Salt;D-Luciferin (sodium salt);D-Luciferin Sodium;D-萤光素钠盐;D-荧光素钠

溶解度

DMSO	95.0 mg/mL (314.3 mM) warming
Water	30.0 mg/mL (99.2 mM)

动物实验配方

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